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YsxC, a putative GTP-binding protein essential for growth of Bacillus subtilis 168

Lookup NU author(s): Dr Zoltan Pragai, Professor Colin Harwood

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Abstract

YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is essential for growth, we attempted to construct mutants in which ysxC was either inactivated or placed under the control of an inducible promoter. Viable mutants were obtained only in the latter case, and these were inducer dependent, demonstrating unambiguously that ysxC is an essential gene.

Publication metadata

Author(s): Pragai Z; Harwood CR

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2000

Volume: 182

Issue: 23

Pages: 6819-6823

ISSN (print): 0021-9193

ISSN (electronic): 1098-5530

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/JB.182.23.6819-6823.2000

DOI: 10.1128/JB.182.23.6819-6823.2000

PubMed id: 11073929

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