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Lookup NU author(s): Dr Sergey Savelev, Dr Ed Okello, Dr Richard WilkinsORCiD, Emeritus Professor Elaine Perry
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In vitro anticholinesterase activities of eight commercially available terpenoid constituents of Salvia lavandulaefolia have been investigated. These included 1,8-cineole, camphor, α-pinene, β-pinene, borneol, caryophyllene oxide, linalool and bornyl acetate. Dose-dependent inhibition of acetylcholinesterase (AChE) by these chemical constituents was determined using the method of Ellman [Biochem. Pharmacol. 7 (1961) 88]. The IC50 value of 1,8-cineole was 0.06±0.01 mg/ml similar to that of the essential oil (0.05±0.01 mg/ml). Analyses of the expected inhibitions based on the prediction of a zero interactive response of a combination at its naturally occurring ratios were carried out in comparison with observed inhibition. Minor synergy was apparent in 1,8-cineole/α-pinene and 1,8-cineole/caryophyllene oxide combinations, with interaction indexes not exceeding 0.5. In contrast, a combination of camphor and 1,8-cineole was antagonistic with an interaction index of 2. A combination of all eight compounds was zero interactive. A combination of six constituents, excluding 1,8-cineole and camphor, was used to compare the method of expected response of a combination with a method of summation. These findings reveal that the inhibitory activity of the oil results from a complex interaction between its constituents, which produce both synergistic and antagonistic responses between the component terpenes. Understanding such interactions is important in comparing species on the basis of chemical composition. © 2003 Elsevier Science Inc. All rights reserved.
Author(s): Savelev S, Okello E, Perry NSL, Wilkins RM, Perry EK
Publication type: Article
Publication status: Published
Journal: Pharmacology Biochemistry and Behavior
Year: 2003
Volume: 75
Issue: 3
Pages: 661-668
Print publication date: 01/06/2003
ISSN (print): 0091-3057
ISSN (electronic): 1873-5177
Publisher: Elsevier
URL: http://dx.doi.org/10.1016/S0091-3057(03)00125-4
DOI: 10.1016/S0091-3057(03)00125-4
PubMed id: 12895684
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