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Lookup NU author(s): Professor Christopher Dennison
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The axial copper ligand methionine has been replaced by a glutamine in the cupredoxin amicyanin from Paracoccus versutus. Dynamic and structural characteristics of the mutant have been studied in detail using UV/Vis, EPR, NMR, cyclic voltammetry, and isomorphous metal replacement. M99Q amicyanin is a blue copper protein with significant spectral and structural similarities to the other cupredoxins umecyanin, stellacyanin, and M121Q azurin. In addition, the functional properties of M99Q amicyanin, as reflected in the electron self-exchange rate constant and midpoint potential (165 mV), have been assessed and compared to values for M121Q azurin. For the latter protein, the published midpoint potential was corrected to the much lower value of 147 mV at pH 7, I = 0.1 M. These values are very similar to the midpoint potential of stellacyanin, which naturally possesses an axial glutamine ligand and has the lowest reduction potential for a naturally occurring cupredoxin. A remarkable feature of M99Q amicyanin, in the reduced state, is the relatively high pK(a)* value of 7.1 for its His96 ligand.
Author(s): Diederix REM, Canters GW, Dennison C
Publication type: Article
Publication status: Published
Journal: Biochemistry
Year: 2000
Volume: 39
Issue: 31
Pages: 9551-9560
ISSN (print): 0006-2960
ISSN (electronic): 1943-295X
Publisher: American Chemical Society
URL: http://dx.doi.org/10.1021/bi000648o
DOI: 10.1021/bi000648o
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