Lookup NU author(s): Dr Chris Redfern,
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To test the hypothesis that 9-cis-retinoic acid is a ligand for cellular retinoic acid binding protein II (CRABP II), human CRABP II was expressed as a glutathione-S-transferase fusion protein (GST-CRABP II) and a single affinity purification step used to extract it from bacterial lysates. GST-CRABP II bound all trans-retinoic acid with high affinity (K(d) 14.2 +/- 6.5 nM), but 9-cis-retinoic acid bound poorly. These studies suggest that 9-cis-retinoic acid is not a ligand for CRABP II. Their ease of purification makes GST-CRABP fusion proteins useful tools for ligand binding studies with different retinoids.
Author(s): Redfern CPF, Wilson KE
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Print publication date: 01/04/1993
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
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