Lookup NU author(s): Dr Benjamin Chaffey,
Dr Mark Birch,
Professor Jeremy Lakey
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Surface biology aims to observe and control biological processes by combining bio-, surface, and physical chemistry. Self-assembled monolayers (SAM) on gold surfaces have provided excellent methods for nanoscale surface preparation for such studies. However, extension of this work requires the specific immobilization of whole protein domains and the direct incorporation of recombinant proteins into SAM is still problematic. In this study a short random coil peptide has been designed to insert into thioalkane layers by formation of a hydrophobic helix. Surface plasmon resonance (SPR) studies show that specific immobilization via the internal cysteine is achieved. Addition of the peptide sequence to the terminus of a protein at the genetic level enables the production of a range of recombinant fusion-proteins with good yield. SPR shows that the proteins display the same gold-binding behavior as the peptide. It is shown that cell growth control can be achieved by printing the proteins using soft lithography with subsequent infilling with thio-alkanes The expression plasmid is constructed so that any stable protein domain can be easily cloned, expressed, purified and immobilized.
Author(s): Chaffey BT, Mitchell E, Birch MA, Lakey JH
Publication type: Article
Publication status: Published
Journal: International Journal of Nanomedicine
Print publication date: 01/01/2008
ISSN (print): 1176-9114
ISSN (electronic): 1178-2013
Publisher: Dove Medical Press Ltd