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Developing a genetic approach to investigate the mechanism of mitochondrial competence for DNA import

Lookup NU author(s): Dr Pierre Boesch, Professor Robert Lightowlers

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Abstract

Mitochondrial gene products are essential for the viability of eukaryote obligate aerobes. Consequently, mutations of the mitochondrial genome cause severe diseases in man and generate traits widely used in plant breeding. Pathogenic mutations can often be identified but direct genetic rescue remains impossible because mitochondrial transformation is still to be achieved in higher eukaryotes. Along this line, it has been shown that isolated plant and mammalian mitochondria are naturally competent for importing linear DNA. However, it has proven difficult to understand how such large polyanions cross the mitochondrial membranes. The genetic tractability of Saccharomyces cerevisae could be a powerful tool to unravel this molecular mechanism. Here we show that isolated S. cerevisiae mitochondria can import linear DNA in a process sharing similar characteristics to plant and mammalian mitochondria. Based on biochemical data, translocation through the outer membrane is believed to be mediated by voltage-dependent anion channel (VDAC) isoforms in higher eukaryotes. Both confirming this hypothesis and validating the yeast model, we illustrate that mitochondria from S. cerevisiae strains deleted for the VDAC-1 or VDAC-2 gene are severely compromised in DNA import. The prospect is now open to screen further mutant yeast strains to identify the elusive inner membrane DNA transporter. (C) 2008 Elsevier B.V. All rights reserved.


Publication metadata

Author(s): Weber-Lotfi F, Ibrahim N, Boesch P, Cosset A, Konstantinov Y, Lightowlers RN, Dietrich A

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Biochimica et Biophysica Acta: Bioenergetics. International Symposium on Mitchondrial Physiology and Pathology

Year of Conference: 2009

Pages: 320-327

Date deposited: 14/06/2010

ISSN: 0005-2728

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.bbabio.2008.11.001

DOI: 10.1016/j.bbabio.2008.11.001

Library holdings: Search Newcastle University Library for this item

ISBN: 00063002


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