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Cob(I)alamin for Trapping Butadiene Epoxides in Metabolism with Rat S9 and for Determining Associated Kinetic Parameters

Lookup NU author(s): Hitesh Motwani, Professor Bernard Golding

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Abstract

The reduced state of vitamin B-12, cob(I)alamin, acts as a supernucleophile that reacts ca. 10(5) times faster than standard nuclephiles, for example, thiols. Methods have been developed for trapping electrophilically reactive Compounds by exploiting this property of cob(I)alamin. 1,3-Butadiene (BD) has recently been classified as a group I human carcinogen by the International Agency for Research on Cancer (IARC). The carcinogenicity of BD is considered to be dependent on the activation or deactivation of the reactive metabolites of BD, that is, the epoxides (oxiranes) 1,2-epoxy-3-butene (EB), 1,13,4-diepoxybutane (DEB), and 1,2-epoxy-3,4-butanediol (EBdiol). Cytochrome P450 (P450) isozymes are involved in oxidation of BD to EB and further activation to DEB. EB and DEB are hydrolyzed by epoxide hydrolases (EH) to 3,4-dihydroxy-1-butene (BDdiol) and EBdiol, respectively. EBdiol can also he formed by oxidation of BDdiol. In the present study, cob(I)alamin was used for instant trapping of the BD epoxide metabolites generated in in vitro metabolism to Study enzyme kinetics. The Substrates EB, DEB. and BDdiol were incubated with rat S9 liver fraction. and apparent K-m and apparent V-max, were determined. The ratio of conversion of EB to DEB (by P450) to the rate of deactivation of DE13 by EH was 1.09. Formation of EBdiol from hydrolysis of DEB was ca. 10 times faster than that from oxidation of BDdiol. It was also found that the oxidation of EB to DEB was much faster than that of BDdiol to EBdiol. The Study offers comparative enzyme kinetic data of different BD metabolic Steps. which is useful for quantitative interspecies comparison. Furthermore, a new application of cob(I)alamin was demonstrated for the measurement of enzyme kinetics Of compounds that form electophilically reactive metabolites.


Publication metadata

Author(s): Motwani HV, Fred C, Hagolund J, Golding BT, Tornqvist M

Publication type: Article

Publication status: Published

Journal: Chemical Research in Toxicology

Year: 2009

Volume: 22

Issue: 9

Pages: 1509-1516

ISSN (print): 0893-228X

ISSN (electronic): 1520-5010

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/tx900088w

DOI: 10.1021/tx900088w


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