Toggle Main Menu Toggle Search

Open Access padlockePrints

Dietary regulation of ruminal bovine UT-B urea transporter expression and localization

Lookup NU author(s): Professor Nicholas Simmons, Dr Abdul Chaudhry, Dr Christopher Graham, Dr Gavin Stewart

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Facilitative UT-B urea transporters have been located in the gastrointestinal tract of numerous mammalian species. We have previously identified UT-B urea transporters within the epithelial layers of the bovine (b) rumen. The aim of this study was to test the hypothesis that ruminal bUT-B urea transporters are regulated by dietary intake. Six Limousine-cross steers (initial BW = 690 +/- 51 kg) were separated into 2 groups fed a basic silage-based diet (RS) or a concentrate-based diet (RC) for 37 d and compared for ruminal morphology, content, and bUT-B expression. Analysis by reverse transcription-PCR showed that ruminal bUT-B2 mRNA expression was greater in RC-fed than RS-fed animals. Utilizing an anti-bUT-B antibody, we also detected a significant increase in bUT-B2 protein expression in RC-fed rumen (P


Publication metadata

Author(s): Simmons NL, Chaudhry AS, Graham C, Scriven ES, Thistlethwaite A, Smith CP, Stewart GS

Publication type: Article

Publication status: Published

Journal: Journal of Animal Science

Year: 2009

Volume: 87

Issue: 10

Pages: 3288-3299

ISSN (print): 0021-8812

ISSN (electronic): 1525-3163

Publisher: American Society of Animal Science

URL: http://dx.doi.org/10.2527/jas.2008-1710

DOI: 10.2527/jas.2008-1710


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share