Lookup NU author(s): Dr Stephan Gruber,
Professor Jeff Errington
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Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and SpoOJ, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which SpoOJ binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large region around the origin of replication depends on the presence of SpoOJ. We show that SpoOJ performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment. Our results demonstrate a direct functional interaction between two widely conserved systems involved in chromosome replication and segregation.
Author(s): Gruber S, Errington J
Publication type: Article
Publication status: Published
ISSN (print): 0092-8674
ISSN (electronic): 1097-4172
Publisher: Cell Press
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