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Umbilical cord blood processing using Prepacyte-CB increases haematopoietic progenitor cell availability over conventional Hetastarch separation

Lookup NU author(s): Christina Basford, Dr Nicolas Forraz, Dr Saba Habibollah, Professor Colin McGuckin

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Abstract

Background: Currently the most frequently used method for umbilical cord blood separation in many hospitals across the UK and the rest of the world, where small-to-medium amounts of samples are processed, is Hetastarch, a mechanical, starch-based method, which causes red cell agglutination by rouleaux formation. Aim: In this study, a novel method (Prepa-Cyte-CB), in comparison with Hetastarch as part of an FDA-approved clinical study, was evaluated. Materials and Methods: Validation of data included recovery of nucleated and CD34+ cells, red blood cell reduction, colony forming unit potential, flow cytometric analysis and sterility tests. Results: PrepaCyte-CB, in comparison with Hetastarch offers fast, reliable separation with improved recovery of nucleated cells, 72.03% (+/- 8.48 SD) compared to 58.09% (+/- 20.06 SD), and CD34+ haematopoietic progenitor cells, 76% (+/- 19.54 SD) compared to 64.19% (+/- 29.77 SD). PrepaCyte-CB was also 12-fold more efficient in removing red blood cells and haemoglobin (P < 0.001) than Hetastarch. Conclusions: These results show that PrepaCyte-CB offers superior separation of UCB when compared to Hetastarch.


Publication metadata

Author(s): Basford C, Forraz N, Habibollah S, Hanger K, McGuckin CP

Publication type: Article

Publication status: Published

Journal: Cell Proliferation

Year: 2009

Volume: 42

Issue: 6

Pages: 751-761

ISSN (print): 0960-7722

ISSN (electronic): 1365-2184

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1111/j.1365-2184.2009.00646.x

DOI: 10.1111/j.1365-2184.2009.00646.x


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