Toggle Main Menu Toggle Search

Open Access padlockePrints

Simple Detection of Protein Soft Structure Changes

Lookup NU author(s): Dr David Allen Chalton, Professor Jeremy Lakey

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

We present a rapid method for protein tertiary structure analysis which avoids the need for techniques such as circular dichroism and differential scanning calorimetry. Small changes to a protein's noncovalent "soft" structure are detected by exploiting differences in thermal stability and fluorescent reporter binding. It can detect subtle stability differences using micrograms of protein in 2 mu L volumes within minutes.


Publication metadata

Author(s): Chalton DA, Lakey JH

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 2010

Volume: 82

Issue: 7

Pages: 3073-3076

Print publication date: 01/04/2010

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society

URL: http://dx.doi.org/10.1021/ac902932c

DOI: 10.1021/ac902932c


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share