Lookup NU author(s): Dr David Allen Chalton,
Professor Jeremy Lakey
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We present a rapid method for protein tertiary structure analysis which avoids the need for techniques such as circular dichroism and differential scanning calorimetry. Small changes to a protein's noncovalent "soft" structure are detected by exploiting differences in thermal stability and fluorescent reporter binding. It can detect subtle stability differences using micrograms of protein in 2 mu L volumes within minutes.
Author(s): Chalton DA, Lakey JH
Publication type: Article
Publication status: Published
Journal: Analytical Chemistry
Print publication date: 01/04/2010
ISSN (print): 0003-2700
ISSN (electronic): 1520-6882
Publisher: American Chemical Society
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