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Knockout of the Bcmo1 gene results in an inflammatory response in female lung, which is suppressed by dietary beta-carotene

Lookup NU author(s): Professor Georg Lietz, Rachel Godschalk

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Abstract

Beta-carotene 15,15'-monooxygenase 1 knockout (Bcmo1 (-/-)) mice accumulate beta-carotene (BC) similarly to humans, whereas wild-type (Bcmo1 (+/+)) mice efficiently cleave BC. Bcmo1 (-/-) mice are therefore suitable to investigate BC-induced alterations in gene expression in lung, assessed by microarray analysis. Bcmo1 (-/-) mice receiving control diet had increased expression of inflammatory genes as compared to BC-supplemented Bcmo1 (-/-) mice and Bcmo1 (+/+) mice that received either control or BC-supplemented diets. Differential gene expression in Bcmo1 (-/-) mice was confirmed by real-time quantitative PCR. Histochemical analysis indeed showed an increase in inflammatory cells in lungs of control Bcmo1 (-/-) mice. Supported by metabolite and gene-expression data, we hypothesize that the increased inflammatory response is due to an altered BC metabolism, resulting in an increased vitamin A requirement in Bcmo1 (-/-) mice. This suggests that effects of BC may depend on inter-individual variations in BC-metabolizing enzymes, such as the frequently occurring human polymorphisms in BCMO1.


Publication metadata

Author(s): van Helden YGJ, Heil SG, van Schooten FJ, Kramer E, Hessel S, Amengual J, Ribot J, Teerds K, Wyss A, Lietz G, Bonet ML, von Lintig J, Godschalk RWL, Keijer J

Publication type: Article

Publication status: Published

Journal: Cellular and Molecular Life Sciences

Year: 2010

Volume: 67

Issue: 12

Pages: 2039-2056

Print publication date: 01/06/2010

ISSN (print): 1420-682X

ISSN (electronic): 1420-9071

Publisher: Birkhaeuser Verlag AG

URL: .http;//dx.doi.org/10.1007/s00018-010-0341-7

DOI: 10.1007/s00018-010-0341-7


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