Toggle Main Menu Toggle Search

Open Access padlockePrints

The stressosome: molecular architecture of a signalling hub

Lookup NU author(s): Dr Jon Marles-Wright, Professor Rick Lewis

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The stressosome co-ordinates the response of Bacillus subtilis to the imposition of a variety of physical and environmental insults. These stresses include fluctuations in salt concentration, the presence of ethanol, changes in pH and even the level of UV light. Despite the obvious and significant differences between these quite different physicochemical stimuli, the result is the same: the stressosome is phosphorylated by a key kinase to initiate the sigma(B) cascade. The phosphorylation of the stressosome initiates a signal transduction system that up-regulates the expression of stress-responsive genes so that the Bacillus can survive the imposition of stress. Hence the stressosome acts as a hub, receiving manifold different stimuli to effect a single outcome. Using single-particle analysis of cryo-electron micrographs, we have been able to reconstruct a series of molecular envelopes of the stressosome. These maps have been interpreted at near-atomic resolution with crystal structures of the individual components of the stressosome to provide the first visualization of this unique signalling hub. The macromolecular structure adopted by the stressosome provides the signalling cascade with the potential for co-operative behaviour, which we have also measured in live bacteria. These experiments are consistent with the tuning of the response of B. subtilis to stress relative to the magnitude of the insult.


Publication metadata

Author(s): Marles-Wright J, Lewis RJ

Publication type: Article

Publication status: Published

Journal: Biochemical Society Transactions

Year: 2010

Volume: 38

Pages: 928-933

Print publication date: 01/08/2010

ISSN (print): 0300-5127

ISSN (electronic): 1470-8752

Publisher: Portland Press

URL: http://dx.doi.org/10.1042/BST0380928

DOI: 10.1042/BST0380928


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share