Direct adsorption of chemically modified biomolecules onto gold: A rapid method for biological functionalization of mems

Suárez, G; Ismail, A; Jackson, R; Chang, S; Harris, A; Burdess, J; Hedley, J; McNeil, C

doi:10.1049/ic:20060453

Direct adsorption of chemically modified biomolecules onto gold: A rapid method for biological functionalization of mems

Lookup NU author(s): Dr Guillaume Suarez, Abd Ismail, Dr Richard Jackson, Dr Seung Chang, Dr Alun Harris, Emeritus Professor James Burdess, Dr John Hedley, Emeritus Professor Calum McNeil

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Abstract

The present work reports a simple, rapid and versatile strategy for glycoprotein immobilization, based on the targeted functionalization of carbohydrate residues with disulphide "anchors" derivatives able to spontaneously chemisorb onto gold, with no need of surface pre- functionalization. Different glycoproteins as enzymes and antibodies were chemically modified using this "one step" bio-immobilization protocol and directly adsorbed onto gold coated interfaces. Surface plasmon resonance (SPR) and Electrochemistry techniques revealed that direct adsorption of a modified glyco-enzyme (horseradish peroxidase) onto gold led to the formation of a densely packed SAM, where each single biomolecule is several thousand times more active than a non modified one. In the case of antibodies, the specific location of the carbohydrate moieties in the constant region resulted in the formation of active and site-oriented SAMs. This bioimmobilization method was applied to the biofunctionalization of a degenerate mode MEMS sensor (MEMSens). Preliminary results obtained for the detection of protein S-100ββ (brain injury marker), indicate a promising low detection limit. © The Institute of Engineering and Technology.