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Alternative splicing of hMLH1 in childhood acute lymphoblastic leukaemia and characterisation of the variably expressed Δ9/10 isoform as a dominant negative species

Lookup NU author(s): Dr Amy Peasland, Elizabeth Matheson, Dr Andrew Hall, Professor Julie Irving

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Abstract

Mismatch repair (MMR) deficiency is a common feature of acute lymphoblastic leukaemia (ALL) cell lines and in some cases is due to the mutations of hMLH1 which affect mRNA splicing. Therefore, we have analysed alternative splicing of hMLH1 in a cohort of children with ALL. We show that alternative splicing of hMLH1 is highly variable in normal and leukaemic cells and can occur by exon skipping or by the use of an alternative splice site, both serving to down-regulate the amount of full-length hMLH1 mRNA/protein produced. Aberrant splicing was found in one child with an aggressive leukaemia in which there was a predominant hMLH1Δ6 form and an associated loss of wild-type hMLH1 protein but this was not accompanied by microsatellite instability. Functional analysis of one of the most abundant spliced forms, hMLH1Δ9/10, was shown to have a significant dominant negative effect on the functionality of the MMR pathway but again was similarly expressed in ALL and normal cells. © 2009 Elsevier Ltd.

Publication metadata

Author(s): Peasland A, Matheson E, Hall A, Irving J

Publication type: Article

Publication status: Published

Journal: Leukemia Research

Year: 2010

Volume: 34

Issue: 3

Pages: 322-327

ISSN (print): 0145-2126

ISSN (electronic): 1873-5835

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/j.leukres.2009.08.015

DOI: 10.1016/j.leukres.2009.08.015

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