Browse by author
Lookup NU author(s): Professor David Graham
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Previous investigations have shown that bacteria can derive benefits from elements within mineral assemblages associated with/or proximal to their point of attachment; however to date, no study has shown a direct connection between mineral dissolution and the biological oxidation of the greenhouse gas methane (CH4). Here we investigate linkages between the Cu content of silicate glasses (Si-glasses) and CH4 oxidation rates in Methylosinus trichosporium OB3b, a type II methanotroph. A series of batch growth experiments were performed under varying solid phase Cu (0, 80, 200, 400, and 800 ppm Cu) and initial aqueous phase methane concentrations (40, 70, and 130 nM CH4). Under near equilibrium conditions, the release of Cu from the glasses increased in association with M. trichosporium OB3b activity. The acquisition of Cu from the glasses directly influenced CH4 oxidation rates of M. trichosporium OB3b, presumably by promoting the expression and activity of copper-containing particulate methane monooxygenase (pMMO), themost efficientMMOformethane oxidation produced. Highest CH4 oxidation rates were observed in glasses with 80 ppm Cu. However, rates generally displayed an inverted-U relationship with solid-phase Cu concentration; i.e., rates were lower with solid phase Cu concentrations < 80 ppm or > 200 ppm. We conclude that in situ methanotroph activity in soils and sediments is strongly influenced by ambient solid-phase Cu concentrations, which has important implications to interpreting greenhouse gas flux data in geologic settings.
Author(s): Kulczycki E, Fowle DA, Kenward PA, Leslie K, Graham DW, Roberts JA
Publication type: Article
Publication status: Published
Journal: Geomicrobiology Journal
Print publication date: 01/01/2011
ISSN (print): 0149-0451
ISSN (electronic): 1521-0529
Publisher: Taylor & Francis Inc.
Altmetrics provided by Altmetric