Toggle Main Menu Toggle Search

Open Access padlockePrints

Evidence of severe mitochondrial oxidative stress and a protective effect of low oxygen in mouse models of inherited photoreceptor degeneration

Lookup NU author(s): Professor Robert Taylor, Emeritus Professor Doug Turnbull

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The role of oxidative stress within photoreceptors (PRs) in inherited photoreceptor degeneration (IPD) is unclear. We investigated this question using four IPD mouse models (Pde6b(rd1/rd1), Pde6b(atrd1/atrd1), Rho(-/-) and Prph2(rds/rds)) and compared the abundance of reduced glutathione (GSH) and the activity of mitochondrial NADH:ubiquinone oxidoreductase (complex I), which is oxidative stress sensitive, as indirect measures of redox status, in the retinas of wild type and IPD mice. All four IPD mutants had significantly reduced retinal complex I activities (14-29% of wild type) and two showed reduced GSH, at a stage prior to the occurrence of significant cell death, whereas mitochondrial citrate synthase, which is oxidative stress insensitive, was unchanged. We orally administered the mitochondrially targeted anti oxidant MitoQ in order to reduce oxidative stress but without any improvement in retinal complex I activity, GSH or rates of PR degeneration. One possible source of oxidative stress in IPDs is oxygen toxicity in the outer retina due to reduced consumption by PR mitochondria. We therefore asked whether a reduction in the ambient O-2 concentration might improve PR survival in Pde6b(rd1/rd1) retinal explants either directly, by reducing reactive oxygen species formation, or indirectly by a neuroprotective mechanism. Pde6b(rd1/rd1) retinal explants cultured in 6% O-2 showed 31% less PR death than normoxic explants. We conclude that (i) mitochondrial oxidative stress is a significant early feature of IPDs; (ii) the ineffectiveness of MitoQ may indicate its inability to reduce some mediators of oxidative stress, such as hydrogen peroxide; and (iii) elucidation of the mechanisms by which hypoxia protects mutant PRs may identify novel neuroprotective pathways in the retina.


Publication metadata

Author(s): Vlachantoni D, Bramall AN, Murphy MP, Taylor RW, Shu XH, Tulloch B, Van Veen T, Turnbull DM, McInnes RR, Wright AF

Publication type: Article

Publication status: Published

Journal: Human Molecular Genetics

Year: 2011

Volume: 20

Issue: 2

Pages: 322-335

Print publication date: 01/01/2011

ISSN (print): 0964-6906

ISSN (electronic): 1460-2083

Publisher: Oxford University Press

URL: http://dx.doi.org/10.1093/hmg/ddq467

DOI: 10.1093/hmg/ddq467


Altmetrics

Altmetrics provided by Altmetric


Funding

Funder referenceFunder name
British Retinitis Pigmentosa Society
Fight for Sight (UK)
Medical Research Council (UK)
Canadian Genetic Diseases Network
Foundation Fighting Blindness of Canada
Macula Vision Research Foundation
MOP-7315Canadian Institutes of Health Research (ICHR)
IOP 54037Canadian Institutes of Health Research (ICHR)

Share