Lookup NU author(s): Christine Aldridge,
Dr Jonathan Brown,
Dr Phillip Aldridge
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
The flagellar genes in Salmonella enterica are expressed in a temporal hierarchy that mirrors the assembly process itself. The sigma(28)-FlgM regulatory circuit plays a key role in controlling this temporal hierarchy. This circuit ensures that the class 3 genes are expressed only when the hook-basal body (HBB), a key intermediate in flagellar assembly, is complete. In this work, we investigated the role of the sigma(28)-FlgM regulatory circuit in controlling the timing and magnitude of class 3 gene expression using a combination of mathematical modelling and experimental analysis. Analysis of the model predicted that this circuit continuously controls class 3 gene expression in response to HBB abundance. We experimentally validated these predictions by eliminating different components of the sigma(28)-FlgM regulatory system and also by rewiring the transcriptional hierarchy. Based on these results, we conclude that the sigma(28)-FlgM regulatory circuit continuously senses the HBB assembly process and regulates class 3 gene expression and possibly flagellar numbers in response.
Author(s): Saini S, Floess E, Aldridge C, Brown J, Aldridge PD, Rao CV
Publication type: Article
Publication status: Published
Journal: Molecular Microbiology
Print publication date: 17/11/2010
ISSN (print): 0950-382X
ISSN (electronic): 1365-2958
Publisher: Wiley-Blackwell Publishing Ltd.
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