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Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite

Lookup NU author(s): Tristano Bacchetti De Gregoris, Professor Grant Burgess, Professor Tony Clare

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Abstract

In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement.


Publication metadata

Author(s): Bacchetti De Gregoris T, Rupp O, Klages S, Knaust F, Bekel T, Kube M, Burgess JG, Arnone MI, Goesmann A, Reinhardt R, Clare AS

Publication type: Article

Publication status: Published

Journal: Biofouling

Year: 2011

Volume: 27

Issue: 4

Pages: 367-374

Print publication date: 26/04/2011

ISSN (print): 0892-7014

ISSN (electronic): 1026-7867

Publisher: Taylor & Francis

URL: http://dx.doi.org/10.1080/08927014.2011.577211

DOI: 10.1080/08927014.2011.577211


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