Toggle Main Menu Toggle Search

Open Access padlockePrints

A practical implementation and exploitation of ATPS for intensive processing of biological feedstock: A novel approach for heavily biological feedstock loaded ATPS

Lookup NU author(s): Professor Andy Lyddiatt

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Aqueous two-phase systems (ATPS) are sensitive to loading with more than modest quantities (>5%, w/w, of system) of biological feedstock due to the contribution of contained macromolecular components (additional to phase-forming chemicals) to the phase diagram in respect of the binodal, tie-line length (TLL) and volume ratio. The present work demonstrates the method in the development and monitoring of ATPS loaded with high concentrations of citrated bovine blood (10-70%, w/w, of system) characterised by varied states of lysis. Variation of protein concentration and extent of cell debris could be accounted for in terms of effective tie-line length (TLLe) values determined by distribution analysis of radiolabelled analytes (DARA) and accommodated by adjustments to phase chemical compositions. The method also provided an opportunity for careful design and control of ATPS formed by adding blood feedstocks to previously monophasic systems (i.e. below the binodal) where phenomena associated with interfacial tension and additional phase formation is sensitively balanced. Such a monophasic/biphasic transformation, uniquely initiated by the phase forming properties of the biological feedstock, facilitates a reduction in the inventory of phase-forming chemicals required to achieve separations. © 2010 Elsevier B.V. All rights reserved.


Publication metadata

Author(s): Selvakumar P, Ling T, Walker S, Lyddiatt A

Publication type: Article

Publication status: Published

Journal: Separation and Purification Technology

Year: 2010

Volume: 75

Issue: 3

Pages: 323-331

Print publication date: 03/09/2010

ISSN (print): 1383-5866

ISSN (electronic): 1873-3794

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/j.seppur.2010.08.022

DOI: 10.1016/j.seppur.2010.08.022


Altmetrics

Altmetrics provided by Altmetric


Share