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Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications

Lookup NU author(s): Professor Eileen Yu

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Abstract

In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.

Publication metadata

Author(s): Yu EH, Prodanovic R, Guven G, Ostafe R, Schwaneberg U

Publication type: Article

Publication status: Published

Journal: Applied Biochemistry and Biotechnology

Year: 2011

Volume: 165

Issue: 7-8

Pages: 1448-1457

Print publication date: 13/09/2011

ISSN (print): 0273-2289

ISSN (electronic): 1559-0291

Publisher: Humana Press, Inc.

URL: http://dx.doi.org/10.1007/s12010-011-9366-0

DOI: 10.1007/s12010-011-9366-0

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