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Molecular design of a splicing switch responsive to the RNA binding protein Tra2β

Lookup NU author(s): Dr Sushma Grellscheid, Caroline Dalgliesh, Dr Agata Rozanska, Professor David Elliott

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Abstract

Tra2β regulates a number of splicing switches including activation of the human testis-specific exon HIPK3-T in the Homeodomain Interacting Protein Kinase 3 gene. By testing HIPK3-T exons of different intrinsic strengths, we found Tra2β most efficiently activated splicing inclusion of intrinsically weak exons, although these were spliced at a lower overall level. Both the RRM and N-terminal RS-rich region of Tra2β were required for splicing activation. Bioinformatic searches for splicing enhancers and repressors mapped four physically distinct exonic splicing enhancers (ESEs) within HIPK3-T, each containing the known Tra2β AGAA-rich binding site. Surprisingly disruption of each single ESE prevented Tra2β-mediated activation, although single mutated exons could still bind Tra2β protein by gel shifts and functional splicing analyses. Titration experiments indicate an additive model of HIPK3-T splicing activation, requiring availability of an array of four distinct ESEs to enable splicing activation. To enable this efficient Tra2β-mediated splicing switch to operate, a closely adjacent downstream and potentially competitive stronger 5'-splice site is actively repressed. Our data indicate that a novel arrangement of multiple mono-specific AGAA-rich ESEs coupled to a weak 5'-splice site functions as a responsive gauge. This gauge monitors changes in the specific nuclear concentration of the RNA binding protein Tra2β, and co-ordinately regulates HIPK3-T exon splicing inclusion.


Publication metadata

Author(s): Grellscheid SN, Dalgliesh C, Rozanska A, Grellscheid D, Bourgeois CF, Stevenin J, Elliott DJ

Publication type: Article

Publication status: Published

Journal: Nucleic Acids Research

Year: 2011

Volume: 39

Issue: 18

Pages: 8092-8104

Print publication date: 01/10/2011

Date deposited: 07/02/2012

ISSN (print): 0305-1048

ISSN (electronic): 1362-4962

Publisher: Oxford University Press

URL: http://dx.doi.org/10.1093/nar/gkr495

DOI: 10.1093/nar/gkr495


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