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Integrating DNA with semiconductor materials: Bio-inorganic hybrid devices

Lookup NU author(s): Dr Andrew Pike, Lars Lie, Dr Samson Patole, Lyndsey Ryder, Professor Bernard Connolly, Dr Ben Horrocks, Professor Andrew Houlton

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Abstract

A method for the automated solid-phase synthesis of DNA on a semiconductor chip with the potential for photolithography to fabricate hybrid electronic-DNA devices was developed. The on-chip oligonucleotide synthetic quality was comparable to standard CPG supports as confirmed by HPLC and gel electrophoresis. Enzymatic manipulation of the immobilised ssDNA was possible by radiolabelling with T4 polynucleotide kinase. Spatial control, afforded by photolithography, was visualised by phosphorimaging radiolabelled dsDNA. The charge transfer properties of DNA were investigated by the association of Ru[(NH3)(6)](3+) with the phosphate backbone and by intercalation with redox active methylene blue. Additionally ferrocene modified nucleosides were incorporated into oligonucleotides to act as electronic mediators for charge transfer. Initial investigations into the effect of the redox group on the nucleobase indicated their potential for use as bioelectronic building blocks for incorporation into silicon based molecular systems.


Publication metadata

Author(s): Pike AR, Lie LH, Patole SN, Ryder LC, Connolly BA, Horrocks BR, Houlton A

Editor(s): Fritzsche, W.

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: DNA-Based Molecular Construction: International Workshop on DNA-based Molecular Construction

Year of Conference: 2002

Pages: 3-12

ISSN: 0094-243X

Publisher: American Institute of Physics

Library holdings: Search Newcastle University Library for this item

ISBN: 9780735400955


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