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Abnormal vascular development in zebrafish models for fukutin and FKRP deficiency

Lookup NU author(s): Dr Alasdair Wood, Dr Juliane Mueller, Catherine Jepson, Dr Steven Laval, Professor Hanns Lochmuller, Professor Katherine Bushby, Dr Rita Barresi, Professor Volker Straub

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Abstract

Fukutin and fukutin-related protein (FKRP) are involved in the glycosylation of a-dystroglycan, a key receptor for basement membrane proteins. Aberrant a-dystroglycan glycosylation leads to a broad spectrum of disorders, ranging from limb girdle muscular dystrophy to Walker-Warburg syndrome. This is the first study investigating a role of fukutin and FKRP-mediated glycosylation in angiogenesis. Transgenic zebrafish expressing enhanced green fluorescent protein in blood vessels were treated with morpholino antisense oligonucleotides that blocked the expression of fukutin, FKRP and dystroglycan. All morphant fish showed muscle damage and vascular abnormalities at day 1 post-fertilization. Intersegmental vessels of somites failed to reach the dorsal longitudinal anastomosis and in more severe phenotypes retracted further or were in some cases even completely missing. In contrast, the eye vasculature was distorted in both fukutin and FKRP morphants, but not in dystroglycan morphants or control fish. The eye size was also smaller in the fukutin and FKRP morphants when compared with dystroglycan knockdown fish and controls. In general, the fukutin morphant fish had the most severe skeletal muscle and eye phenotype. Our findings suggest that fukutin and FKRP have functions that affect ocular development in zebrafish independently of dystroglycan. Despite anecdotal reports about vascular abnormalities in patients affected by dystroglycanopathies, the clinical relevance of such lesions remains unclear and should be subject to further review and investigations.


Publication metadata

Author(s): Wood AJ, Muller JS, Jepson CD, Laval SH, Lochmuller H, Bushby K, Barresi R, Straub V

Publication type: Article

Publication status: Published

Journal: Human Molecular Genetics

Year: 2011

Volume: 20

Issue: 24

Pages: 4879-4890

Print publication date: 15/09/2011

ISSN (print): 0964-6906

ISSN (electronic): 1460-2083

Publisher: Oxford University Press

URL: http://dx.doi.org/10.1093/hmg/ddr426

DOI: 10.1093/hmg/ddr426


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