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Lookup NU author(s): Dr Kirsten Wolff
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The use of randomly amplified polymorphic DNA (RAPD) data in studies of population genetic structure and evolution is considered. Two case studies are described to exemplify the use to which RAPD data may be put. In the first, population substructuring and gene flow in an Egyptian population of Alkanna orientalis is investigated, the results of which indicate that the substructuring is related to topographical barriers and that gene flow over distances of more than a few metres is mediated more by flash floods than by pollinators. In the second study, analysis of RAPD variation in Plantago major s.l. has revealed the existence of two species: P. major and P. intermedia, despite their morphological resemblance and absence of breeding barriers. RAPD analysis is a very good starting point for studies of relationships within and among closely related species. When the limitations are kept in mind and the techniques are applied properly RAPDs will often give a quick, easily obtainable and repeatable result, even in the hands of a beginner.
Author(s): Wolff K; Morgan-Richards M
Editor(s): PM Hollingsworth, RM Bateman, RJ Gornall
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Year of Conference: 1999
Publisher: Taylor & Francis, Ltd
Library holdings: Search Newcastle University Library for this item
Series Title: Molecular Systematics and Plant Evolution (Systematics Association Special Volume Series)