Lookup NU author(s): Dr Evan Mulligan,
Dr Natalie Ferry,
Dr Gordon Port,
Professor Angharad MR Gatehouse
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The potential for transgenic oilseed rape (OSR) plants expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) to affect the field slug Deroceras reticulatum was investigated. Characterization of proteolytic digestive enzymes from the slug's digestive gland demonstrated that all age classes of D. reticulatum utilise the OC-1 sensitive cysteine protease, cathepsin-L. Vegetative tissues of transgenic OSR plants were shown to express OC-1 at levels of 0.025-0.1% of total soluble protein. Bio-assays were carried out over a 6 week period to determine the effects of OC-1-expressing OSR on the growth, development and survival of D. reticulatum juveniles; effects on plant damage were also monitored. Despite OC-1 being both a potent inhibitor of digestive proteolysis in vitro and reducing endogenous proteolysis in vivo, slugs in the experimental group performed significantly better in terms of survival and development; furthermore, plant damage was also significantly greater in this group.
Author(s): Mulligan EA, Ferry N, Port G, Gatehouse AMR, Walters K
Editor(s): Dussart, G.B.J.
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: Slugs and Snails: Agricultural, Veterinary and Environmental Perspectives
Year of Conference: 2003
Publisher: British Crop Protection Council
Library holdings: Search Newcastle University Library for this item
Series Title: British Crop Protection Council Symposium Proceedings