Toggle Main Menu Toggle Search

ePrints

Impacts of transgenic crop technology upon the field slug Deroceras reticulatum (Muller)

Lookup NU author(s): Dr Evan Mulligan, Dr Natalie Ferry, Dr Gordon Port, Professor Angharad MR Gatehouse

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The potential for transgenic oilseed rape (OSR) plants expressing the cysteine protease inhibitor oryzacystatin-1 (OC-1) to affect the field slug Deroceras reticulatum was investigated. Characterization of proteolytic digestive enzymes from the slug's digestive gland demonstrated that all age classes of D. reticulatum utilise the OC-1 sensitive cysteine protease, cathepsin-L. Vegetative tissues of transgenic OSR plants were shown to express OC-1 at levels of 0.025-0.1% of total soluble protein. Bio-assays were carried out over a 6 week period to determine the effects of OC-1-expressing OSR on the growth, development and survival of D. reticulatum juveniles; effects on plant damage were also monitored. Despite OC-1 being both a potent inhibitor of digestive proteolysis in vitro and reducing endogenous proteolysis in vivo, slugs in the experimental group performed significantly better in terms of survival and development; furthermore, plant damage was also significantly greater in this group.


Publication metadata

Author(s): Mulligan EA, Ferry N, Port G, Gatehouse AMR, Walters K

Editor(s): Dussart, G.B.J.

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Slugs and Snails: Agricultural, Veterinary and Environmental Perspectives

Year of Conference: 2003

Pages: 121-126

ISSN: 0306-3941

Publisher: British Crop Protection Council

Library holdings: Search Newcastle University Library for this item

Series Title: British Crop Protection Council Symposium Proceedings

ISBN: 9781901396805


Actions

    Link to this publication


Share