Lookup NU author(s): Dr Heather Lamb,
Professor Alastair Hawkins
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MbeA and MbeC are two key proteins in plasmid ColE1 conjugal mobilization. Isothermal titration calorimetry was used to detect and quantify an interaction between MbeA and MbeC. As a result of this interaction, the affinity of MbeA for single stranded DNA increased. The interaction was confirmed in vivo using a bacterial two-hybrid system, which revealed that MbeA-MbeC complexes are formed through the amino-terminal region of MbeA and the carboxy-terminal region of MbeC. To the best of our knowledge, this is the first report of direct interactions between conjugative proteins encoded by a mobilizable plasmid. Structured summary of protein interactions: mbeA and mbeC physically interact by two hybrid (View interaction) mbeA and mbeC bind by isothermal titration calorimetry (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Author(s): Varsaki A, Lamb HK, Eleftheriadou O, Vandera E, Thompson P, Moncalian G, de la Cruz F, Hawkins AR, Drainas C
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Print publication date: 01/03/2012
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: Elsevier BV
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