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Lookup NU author(s): Piero Dalle Pezze,
Dr Daryl Shanley
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We modeled the mammalian or mechanistic target of rapamycin (mTOR) network and proposed a previously unknown mode of activation of the mTOR-containing complex mTORC2 through a phosphoinositide 3-kinase-dependent, and tuberous sclerosis complex-independent mechanism. Manning questions the validity of using the phosphorylation of Ser(2481) of mTOR as a specific readout of mTORC2 activity and suggests an in vitro mTORC2 kinase assay as a more appropriate method to parameterize a dynamic mTOR model. We maintain that our computational-experimental approach in combination with careful selection of the readout and cell system is appropriate for studying mTORC2 regulation by insulin.
Author(s): Dalle Pezze P, Sonntag AG, Shanley DP, Thedieck K
Publication type: Letter
Publication status: Published
Journal: Science Signaling
Print publication date: 10/07/2012
ISSN (print): 1945-0877
ISSN (electronic): 1937-9145
Publisher: AMER ASSOC ADVANCEMENT SCIENCE