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How does Tra2 beta protein regulate tissue-specific RNA splicing?

Lookup NU author(s): Professor David Elliott, Andy Best, Caroline Dalgliesh, Dr Ingrid Ehrmann, Dr Sushma Grellscheid

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Abstract

The splicing regulator protein Tra2 beta is conserved between humans and insects and is essential for mouse development. Recent identification of physiological RNA targets has started to uncover molecular targets and mechanisms of action of Tra2 beta. At a transcriptome-wide level, Tra2 beta protein binds a matrix of AGAA-rich sequences mapping frequently to exons. Particular tissue-specific alternatively spliced exons contain high concentrations of high scoring Tra2 beta-binding sites and bind Tra2 beta strongly in vitro. These top exons were also activated for splicing inclusion in cellulo by co-expression of Tra2 beta protein and were significantly down-regulated after genetic depletion of Tra2 beta. Tra2 beta itself seems to be fairly evenly expressed across several different mouse tissues. In the present paper, we review the properties of Tra2 beta and its regulated target exons, and mechanisms through which this fairly evenly expressed alternative splicing regulator might drive tissue-specific splicing patterns.


Publication metadata

Author(s): Elliott DJ, Best A, Dalgliesh C, Ehrmann I, Grellscheid S

Publication type: Article

Publication status: Published

Journal: Biochemical Society Transactions

Year: 2012

Volume: 40

Pages: 784-788

Print publication date: 01/08/2012

ISSN (print): 0300-5127

ISSN (electronic): 1470-8752

Publisher: Portland Press Ltd.

URL: http://dx.doi.org/10.1042/BST20120036

DOI: 10.1042/BST20120036


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