Toggle Main Menu Toggle Search

ePrints

Back-priming mode of Phi6 RNA-dependent RNA polymerase

Lookup NU author(s): Dr Paula Salgado

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage ϕ6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629–632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3′ terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.


Publication metadata

Author(s): Laurila MRL, Salgado PS, Stuart DI, Grimes JM, Bamford DH

Publication type: Article

Publication status: Published

Journal: Journal of General Virology

Year: 2005

Volume: 86

Issue: 2

ISSN (print): 0022-1317

ISSN (electronic): 1465-2099

Publisher: Society for General Microbiology

URL: http://dx.doi.org/10.1099/vir.0.80492-0

DOI: 10.1099/vir.0.80492-0

PubMed id: 15659773

Notes: Also available at: http://vir.sgmjournals.org/content/86/2/521.long


Altmetrics

Altmetrics provided by Altmetric


Actions

    Link to this publication


Share