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Going soft and SAD with manganese

Lookup NU author(s): Dr Paula Salgado

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Abstract

SAD phasing has been revisited recently, with experiments being carried out using previously unconventional sources of anomalous signal, particularly lighter atoms and softer X-rays. A case study is reported using the 75 kDa RNA-dependent RNA polymerase of the bacteriophase [varphi]6, which binds a Mn atom and crystallizes with three molecules in the asymmetric unit. X-ray diffraction data were collected at a wavelength of 1.89 Å and although the calculated anomalous signal from the three Mn atoms was only 1.2%, SHELXD and SOLVE were able to locate these atoms. SOLVE/RESOLVE used this information to obtain SAD phases and automatically build a model for the core region of the protein, which possessed the characteristic features of the right-hand polymerase motif. These results demonstrate that with modern synchrotron beamlines and software, manganese phasing is a practical tool for solving the structure of large proteins.


Publication metadata

Author(s): Salgado PS, Walsh MA, Laurila MRL, Stuart DI, Grimes JM

Publication type: Article

Publication status: Published

Journal: Acta Biol Crystallographica D

Year: 2005

Volume: D61

Issue: 1

Pages: 108-111

ISSN (print): 0907-4449

ISSN (electronic): 1399-0047

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1107/S0907444904026800

DOI: 10.1107/S0907444904026800

PubMed id: 15608382


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