Lookup NU author(s): Dr Paula Salgado
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The RNA-dependent RNA polymerase, QDE-1, is a component of the RNA silencing pathway in Neurospora crassa. The enzymatically active carboxy-terminal fragment QDE-1 ΔN has been expressed in Saccharomyces cerevisiae in the presence and absence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by mass spectrometry to be ∼98%. Both native and SeMet proteins were crystallized in space group P21 with unit cell parameters a = 101.2, b = 122.5, c = 114.4 Å, β = 108.9°, and 2 molecules per asymmetric unit. The native and SeMet crystals diffract to 2.3 and 3.2 Å, respectively, the latter are suitable for MAD structure determination.
Author(s): Laurila MR, Salgado PS, Makeyev EV, Nettelship J, Stuart DI, Grimes JM, Bamford DH
Publication type: Article
Publication status: Published
Journal: Journal of Structural Biology
ISSN (print): 1047-8477
ISSN (electronic): 1095-8657
Publisher: Academic Press
PubMed id: 15629662
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