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Optimised Protocols for the Identification of the Murine Cardiac Side Population

Lookup NU author(s): Dr Annette Meeson, Andrew Fuller, Dr Andrew Owens, Dr Gavin Richardson

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Abstract

Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype relies on an active metabolic pathway and cell viability to identify a rare population of cells and therefore technical differences in the CSP staining protocol can lead to inconsistent results and discrepancies between studies. Here we describe an established protocol for CSP identification and have optimised a protocol for CSP analysis utilizing an automated cardiac digestion procedure using gentleMACs dissociation and Hoechst 33342 staining followed by dual wavelength flow cytometric analysis.


Publication metadata

Author(s): Meeson A, Fuller A, Breault D, Owens W, Richardson G

Publication type: Article

Publication status: Published

Journal: Stem Cell Reviews and Reports

Year: 2013

Volume: 9

Issue: 5

Pages: 731-739

Print publication date: 26/04/2013

ISSN (print): 1550-8943

ISSN (electronic): 1558-6804

Publisher: Humana Press, Inc.

URL: http://dx.doi.org/10.1007/s12015-013-9440-9

DOI: 10.1007/s12015-013-9440-9


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