Browse by author
Lookup NU author(s): Emeritus Professor Harry Gilbert
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Cellulosomes are highly efficient nanomachines that play a fundamental role during the anaerobic deconstruction of complex plant cell wall carbohydrates. The assembly of these complex nanomachines results from the very tight binding of repetitive cohesin modules, located in a noncatalytic molecular scaffold, and dockerin domains located at the C-terminus of the enzyme components of the cellulosome. The number of enzymes found in a cellulosome varies but may reach more than 100 catalytic subunits if cellulosomes are further organized in poly-cellulosomes, through a second type of cohesin dockerin interaction. Structural studies have revealed how the cohesin dockerin interaction mediates cellulosome assembly and cell-surface attachment, while retaining the flexibility required to potentiate catalytic synergy within the complex. Methods that might be applied for the production, purification, and structure determination of cohesin dockerin complexes are described here.
Author(s): Bras JLA, Carvalho AL, Viegas A, Najmudin S, Alves VD, Prates JAM, Ferreira LMA, Romao MJ, Gilbert HJ, Fontes CMGA
Editor(s): Gilbert, H.J.
Publication type: Book Chapter
Publication status: Published
Book Title: Cellulases
Print publication date: 01/01/2012
Series Title: Methods in Enzymology (ISSN: 0076-6879)
Publisher: Academic Press
Place Published: San Diego, California, USA
Library holdings: Search Newcastle University Library for this item