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Removal of Biofilms from Tracheoesophageal Speech Valves Using a Novel Marine Microbial Deoxyribonuclease

Lookup NU author(s): Mohamed Elbadawey, Robert Shields, Dr Nicholas Jakubovics, Professor Grant Burgess

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Abstract

Objective. The growth of biofilms on tracheoesophageal speech valves shortens their life span and produces a reservoir of pathogens that may infect the respiratory tract. The authors have discovered a novel nontoxic deoxyribonuclease, NucB, from a marine isolate of Bacillus licheniformis that is effective at dispersing a variety of mono and mixed-species bacterial biofilms. The aim of this preliminary study was to determine whether NucB could also disrupt and remove mixed-species biofilms from tracheoesophageal speech valves. Study Design. Laboratory-based treatment and analysis of discarded tracheoesophageal speech valves. Setting. University human biology laboratory and the Department of Speech and Language Therapy at a tertiary referral hospital. Subjects and Methods. Seventeen ex vivo tracheoesophageal speech valves fouled with natural human biofilms were collected and divided into 2 equal parts. One half was treated with NucB and the other half with a control buffer solution. Biofilm removal was measured by microscopy and by culture of dispersed biofilm organisms on agar plates. Results. Significantly more organisms were released from biofilms using NucB than with buffer solution alone. On nonselective medium, more organisms were cultured in 11 samples (65%, n = 17, P < .05). Using growth media favoring fungi, more organisms were cultured in 14 samples (82%, n = 17, P < .05). Conclusion. The nontoxic deoxyribonuclease NucB was effective in releasing more microorganisms from biofilms on tracheoesophageal speech valves. This reflects its potential ability to break up and disperse these biofilms. Future studies will aim to develop NucB as a novel agent to prolong the life span of tracheoesophageal speech valves, thus reducing health care costs.


Publication metadata

Author(s): Shakir A, ElBadawey MR, Shields RC, Jakubovics NS, Burgess JG

Publication type: Article

Publication status: Published

Journal: Otolaryngology: Head and Neck Surgery

Year: 2012

Volume: 147

Issue: 3

Pages: 509-514

Print publication date: 02/04/2012

ISSN (print): 0194-5998

ISSN (electronic): 1097-6817

Publisher: Sage Publications, Inc.

URL: http://dx.doi.org/10.1177/0194599812442867

DOI: 10.1177/0194599812442867


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