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Mouse epidermal neural crest stem cell (EPI-NCSC) cultures

Lookup NU author(s): Professor Maya Sieber-Blum

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Abstract

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the physiological property to generate a wide array of differentiated cell types, including neurons, nerve supporting cells, smooth muscle cells, bone/cartilage cells and melanocytes. EPI-NCSC are easily accessible in the hairy skin and can be isolated as a highly pure population of stem cells. This video provides a detailed protocol for preparing mouse EPI-NCSC cultures from whisker follicles. The whisker pad of an adult mouse is removed, and whisker follicles dissected. The follicles are then cut longitudinally and subsequently transversely above and below the bulge region. The bulge is removed from the collagen capsule and placed in a culture plate. EPI-NCSC start to emigrate from the bulge explants 3 to 4 days later.


Publication metadata

Author(s): Sieber-Blum M, Hu YF

Publication type: Article

Publication status: Published

Journal: Journal of Visualized Experiments

Year: 2013

Volume: 15

Print publication date: 09/05/2013

ISSN (electronic): 1940-087X

Publisher: Journal of Visualized Experiments

URL: http://dx.doi.org/10.3791/772

DOI: 10.3791/772

Notes: The video component of this article can be found at: http://www.jove.com/video/772/ video publication: 23,192 views


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