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Polyunsaturated fatty acid production by marine bacteria

Lookup NU author(s): Ahmed Abd Elrazak, Emeritus Professor Alan Ward, Professor Jarka Glassey

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Abstract

Polyunsaturated fatty acids are important in maintaining human health. Limitations associated with current sources of ω-3 fatty acids and ω-6 fatty acids, from animal and plant sources, have led to increased interest in microbial production. Marine bacteria may provide a suitable alternative, although the isolation of production strains and the identification of operating conditions must be addressed before manufacturing processes become economically viable. Marine isolate 560 was identified as an eicosapentaenoic acid (EPA) producer via GC/MS. The isolate was initially identified as Vibrio cyclitrophicus by 16S rRNA sequencing. Statistically based experimental designs were applied to the optimisation of medium components and environmental factors for the production of EPA. A Plackett–Burman design was used to screen for the effect of temperature, pH, and media components. Subsequently, the concentrations of NaCl, yeast extract, and peptone, identified as significant factors, were optimised using a central composite design. The predicted optimal combination of media components for maximum EPA production (4.8 mg/g dry weight) was determined as 7.9 g/l peptone, 16.2 g/l NaCl, and 6.2 g/l yeast extract. On transfer of this process to bioreactor cultivation, where a range of pH and DO values were tested, the maximum amount of EPA produced increased to 7.5 mg/g dry weight and 10 % of the total fatty acid.


Publication metadata

Author(s): Abd Elrazak A, Ward AC, Glassey J

Publication type: Article

Publication status: Published

Journal: Bioprocess and Biosystems Engineering

Year: 2013

Volume: 36

Issue: 11

Pages: 1641-1652

Print publication date: 01/11/2013

Online publication date: 23/03/2013

ISSN (print): 1615-7591

ISSN (electronic): 1615-7605

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00449-013-0936-0

DOI: 10.1007/s00449-013-0936-0


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