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MicroRNAs are suitable for assessment as biomarkers from formalin-fixed paraffin-embedded tissue, and miR-24 represents an appropriate reference microRNA for diffuse large B-cell lymphoma studies

Lookup NU author(s): Dr Rachel Crossland, Dr Michal Sieniawski, Parimala Saminathan, Dr Tryfonia Mainou-Fowler

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Abstract

Tissue biopsy specimens in the form of formalin-fixed paraffin-embedded tissue (FFPET) represent a valuable resource for biomarker identification and validation. However, to date, they remain an underused asset due to uncertainty regarding RNA extraction and the reliability of downstream techniques, including quantitative RT-PCR. Recently, much interest has emerged in the study of microRNAs; small single-stranded RNAs with a role in transcriptional regulation, that are thought to be well preserved in FFPET. In this study, we show that microRNA expression is comparable between FFPET and matched fresh-frozen samples (miR-17-5p: p=0.01, miR-92: p=0.003), and demonstrate that no significant deterioration in expression occurs over prolonged FFPET storage (p=0.06). Furthermore, microRNA expression is equivalent dependant on RNA extraction method (p<0.001) or DNAse treatment of total RNA (p<0.001). Finally, we validate miR-24 as a suitable reference microRNA for diffuse large B-cell lymphoma (DLBCL) FFPET studies.


Publication metadata

Author(s): Culpin RE, Sieniawski M, Proctor SJ, Menon G, Mainou-Fowler T

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Pathology

Year: 2013

Volume: 66

Issue: 3

Pages: 249-252

Print publication date: 01/03/2013

ISSN (print): 0021-9746

ISSN (electronic):

Publisher: BMJ Group

URL: http://dx.doi.org/10.1136/jclinpath-2012-201021

DOI: 10.1136/jclinpath-2012-201021


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