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Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture

Lookup NU author(s): Dr Aaron Gardner, Dr Andrew Owens

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Abstract

Traditional skin grafting techniques are effective but limited methods of skin replacement. Autologous transplantation of rapidly cultured keratinocytes is successful for epidermal regeneration, but the current gold-standard technique requires mouse fibroblast feeders and serum-rich media, with serum-free systems and dermal fibroblast (DF) feeders performing relatively poorly. Here, we investigated the capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth. Dermal papilla (DP) dermal sheath (DS), DF and 3T3 cells were used as inactivated feeder cells for human keratinocyte coculture. Under conditions favouring dermal cells, proliferation of keratinocytes in the presence of either DS or DP cells was significantly enhanced compared with DF cells, at levels comparable to keratinocytes cultured under gold-standard conditions. Secreted protein acidic and rich in cysteine (SPARC) expression increased DS and DP cells relative to DFs; however, further experiments did not demonstrate a role in keratinocyte support.


Publication metadata

Author(s): Hill RP, Gardner A, Crawford HC, Richer R, Dodds A, Owens WA, Lawrence C, Rao S, Kara B, James SE, Jahoda CA

Publication type: Article

Publication status: Published

Journal: Experimental Dermatology

Year: 2013

Volume: 22

Issue: 3

Pages: 236-238

Print publication date: 01/03/2013

Online publication date: 12/03/2013

Acceptance date: 31/01/2013

ISSN (print): 0906-6705

ISSN (electronic): 1600-0625

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1111/exd.12107

DOI: 10.1111/exd.12107


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