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Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules

Lookup NU author(s): Dr Heiko Peters

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Abstract

Anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the esophagus, salivary glands, lung, thymus, parathyroid and thyroid. Despite its importance, reports describing the generation of AFE from pluripotent stem cells (PSCs) by directed differentiation have mainly focused on the Nkx2.1+ lung and thyroid lineages. Here, we describe a novel protocol to derive a subdomain of AFE, identified by expression of Pax9, from PSCs using small molecules and defined media conditions. We generated a reporter PSC line for isolation and characterization of Pax9+ AFE cells, which when transplanted in vivo, can form several distinct complex AFE-derived epithelia, including mucosal glands and stratified squamous epithelium. Finally, we show that the directed differentiation protocol can be used to generate AFE from human PSCs. Thus, this work both broadens the range of PSC-derived AFE tissues and creates a platform enabling the study of AFE disorders.


Publication metadata

Author(s): Kearns NA, Genga RMJ, Ziller M, Kapina K, Peters H, Brehm MA, Meissner A, Maehr R

Publication type: Article

Publication status: Published

Journal: Stem Cell Research

Year: 2013

Volume: 11

Issue: 3

Pages: 1003-1012

Print publication date: 01/11/2013

ISSN (print): 1873-5061

ISSN (electronic): 1876-7753

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.scr.2013.06.007

DOI: 10.1016/j.scr.2013.06.007


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