Toggle Main Menu Toggle Search

Open Access padlockePrints

SecA is required for membrane targeting of the cell division protein DivIVA in vivo

Lookup NU author(s): Dr Sven Halbedel, Maki Kawai, Dr Leendert Hamoen

Downloads


Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

The conserved protein DivIVA is involved in different morphogenetic processes in Gram-positive bacteria. In Bacillus subtilis, the protein localizes to the cell division site and cell poles, and functions as a scaffold for proteins that regulate division site selection, and for proteins that are required for sporulation. To identify other proteins that bind to DivIVA, we performed an in vivo cross-linking experiment. A possible candidate that emerged was the secretion motor ATPase SecA. SecA mutants have been described that inhibit sporulation, and since DivIVA is necessary for sporulation, we examined the localization of DivIVA in these mutants. Surprisingly, DivIVA was delocalized, suggesting that SecA is required for DivIVA targeting. To further corroborate this, we performed SecA depletion and inhibition experiments, which provided further indications that DivIVA localization depends on SecA. Cell fractionation experiments showed that SecA is important for binding of DivIVA to the cell membrane. This was unexpected since DivIVA does not contain a signal sequence, and is able to bind to artificial lipid membranes in vitro without support of other proteins. SecA is required for protein secretion and membrane insertion, and therefore its role in DivIVA localization is likely indirect. Possible alternative roles of SecA in DivIVA folding and/or targeting are discussed.


Publication metadata

Author(s): Halbedel S, Kawai M, Breitling R, Hamoen LW

Publication type: Article

Publication status: Published

Journal: Frontiers in Microbiology

Year: 2014

Volume: 5

Online publication date: 14/02/2014

Acceptance date: 29/01/2014

Date deposited: 21/05/2014

ISSN (electronic): 1664-302X

Publisher: Frontiers Research Foundation

URL: http://dx.doi.org/10.3389/fmicb.2014.00058

DOI: 10.3389/fmicb.2014.00058


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share