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Regulation of the clpP1clpP2 operon by the pleiotropic regulator AdpA in Streptomyces lividans

Lookup NU author(s): Dr Aurelie Guyet

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Abstract

Insertion of an apramycin resistance cassette in the clpP1clpP2 operon (encoding the ClpP1 and ClpP2 peptidase subunits) affects morphological and physiological differentiation of Streptomyces lividans. Another key factor controlling Streptomyces differentiation is the pleiotropic transcriptional regulator AdpA. We have identified a spontaneous missense mutation (-1 frameshift) in the adpA (bldH) open reading frame in a clpP1clpP2 mutant that led to the synthesis of a non-functional AdpA protein. Electrophoretic mobility shift assays showed that AdpA bound directly to clpP1clpP2 promoter region. Quantitative real-time PCR analysis showed that AdpA regulated the clpP1clpP2 operon expression at specific growth times. In vitro, AdpA and ClgR, a transcriptional activator of clpP1clpP2 operon and other genes, were able to bind simultaneously to clpP1 promoter, which suggests that AdpA binding to clpP1 promoter did not affect that of ClgR. This study allowed to uncover an interplay between the ClpP peptidases and AdpA in S. lividans.

Publication metadata

Author(s): Guyet A, Gominet M, Benaroudj N, Mazodier P

Publication type: Article

Publication status: Published

Journal: Archives of Microbiology

Year: 2013

Volume: 195

Issue: 12

Pages: 831-841

Print publication date: 01/12/2013

Online publication date: 07/11/2013

Acceptance date: 26/07/2013

ISSN (print): 0302-8933

ISSN (electronic): 1432-072X

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00203-013-0918-2

DOI: 10.1007/s00203-013-0918-2

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