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Portal Venous Oxygen Persufflation of the Donation after Cardiac Death pancreas in a rat model is superior to static cold storage and hypothermic machine perfusion

Lookup NU author(s): Professor James Shaw, David Talbot

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Abstract

Success of clinical pancreatic islet transplantation depends on the mass of viable islets transplanted and the proportion of transplanted islets that survive early ischaemia reperfusion injury. Novel pancreas preservation techniques to improve islet preservation and viability can increase the utilization of donation after cardiac death donor pancreases for islet transplantation. Rat pancreases were retrieved after 30min of warm ischaemia and preserved by static cold storage, hypothermic machine perfusion or retrograde portal venous oxygen persufflation for 6h. They underwent collagenase digestion and density gradient separation to isolate islets. The yield, viability, morphology were compared. In vitro function of isolated islets was compared using glucose stimulated insulin secretion test. Portal venous oxygen persufflation improved the islet yield, viability and morphology as compared to static cold storage. The percentage of pancreases with good in vitro function (stimulation index >1.0) was also higher after oxygen persufflation as compared to static cold storage. Retrograde portal venous oxygen persufflation of donation after cardiac death donor rat pancreases has the potential to improve islet yield.


Publication metadata

Author(s): Reddy MS, Carter N, Cunningham A, Shaw J, Talbot D

Publication type: Article

Publication status: Published

Journal: Transplant International

Year: 2014

Volume: 27

Issue: 6

Pages: 634-639

Print publication date: 12/04/2014

ISSN (print): 0934-0874

ISSN (electronic): 1432-2277

Publisher: Wiley-Blackwell Publishing

URL: http://dx.doi.org/10.1111/tri.12313

DOI: 10.1111/tri.12313


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