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Lack of association between KIR and HLA-C type and susceptibility to idiopathic bronchiectasis

Lookup NU author(s): Dr Ali Anwar, Professor Anthony De Soyza, Dr Sylvia Worthy, Professor Paul Corris, Dr James Lordan, Dr Christopher Ward, Dr Peter Middleton

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Abstract

Introduction: Idiopathic bronchiectasis is a poorly defined disease characterised by persistent inflammation, infection and progressive lung damage. Natural killer (NK) cells provide a major defense against infection, through the interaction of their surface receptors, including the activating and inhibitory killer immunoglobulin-like receptors (KIR), and human leukocyte antigens (HLA) class I molecules. Homozygosity for HLA-C has been shown in a single study to confer increased genetic susceptibility to idiopathic bronchiectasis. We aimed to assess whether the KIR and HLA repertoire, alone or in combination, may influence the risk of developing idiopathic bronchiectasis, in an independent replication study.Methods: In this prospective, observational, case-control association study, 79 idiopathic bronchiectasis patients diagnosed following extensive aetiological investigation were compared with 98 anonymous, healthy, age, sex and ethnically-matched controls attending blood donor sessions in the same geographical location. DNA extraction was performed according to standardised techniques. Determination of presence or absence of KIR genes was performed by a sequence specific oligonucleotide probe method. Allele frequencies for the proposed KIR, HLA-B and HLA-C risk alleles both individually and in combinations were compared.Results: We found no significant differences in allele frequency between the idiopathic bronchiectasis and control samples, whether considering HLA-C group homozygosity alone or in combination with the KIR type.Discussion: Our results do not show an association between HLA-C and KIR and therefore do not confirm previous positive findings. This may be explained by the lower frequency of HLA-C1 group homozygosity in the control population of the previous study (27.2%), compared to 42.3% in our study, which is consistent with the genetic profiling of control groups across the UK. The previous positive association study may therefore have been driven by an anomalous control group. Further larger prospective multicentre replication studies are needed to determine if an association exists. (C) 2014 Elsevier Ltd. All rights reserved.


Publication metadata

Author(s): McDonnell MJ, Anwar GA, Rutherford RM, De Soyza A, Worthy S, Corris PA, Lordan JL, Bourke S, Afolabi G, Ward C, Middleton P, Middleton D

Publication type: Article

Publication status: Published

Journal: Respiratory Medicine

Year: 2014

Volume: 108

Issue: 8

Pages: 1127-1133

Print publication date: 01/08/2014

Online publication date: 18/06/2014

Acceptance date: 30/05/2014

ISSN (print): 0954-6111

ISSN (electronic): 1532-3064

Publisher: Elsevier Ltd

URL: http://dx.doi.org/10.1016/j.rmed.2014.05.017

DOI: 10.1016/j.rmed.2014.05.017


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