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Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from sugarcane soil metagenome

Lookup NU author(s): Thabata Alvarez, Professor Harry Gilbert

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Abstract

In recent years, owing to the growing global demand for energy, dependence on fossil fuels, limited natural resources and environmental pollution, biofuels have attracted great interest as a source of renewable energy. However, the production of biofuels from plant biomass is still considered to be an expensive technology. In this context, the study of carbohydrate-binding modules (CBMs), which are involved in guiding the catalytic domains of glycoside hydrolases for polysaccharide degradation, is attracting growing attention. Aiming at the identification of new CBMs, a sugarcane soil metagenomic library was analyzed and an uncharacterized CBM (CBM_E1) was identified. In this study, CBM_E1 was expressed, purified and crystallized. X-ray diffraction data were collected to 1.95 angstrom resolution. The crystals, which were obtained by the sitting-drop vapourdiffusion method, belonged to space group I23, with unit-cell parameters a = b = c = 88.07 angstrom.


Publication metadata

Author(s): Campos BM, Alvarez TM, Liberato MV, Polikarpov I, Gilbert HJ, Zeri ACD, Squina FM

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica Section F: Structural Biology Communications

Year: 2014

Volume: 70

Issue: 9

Pages: 1232-1235

Print publication date: 01/09/2014

Online publication date: 27/08/2014

Acceptance date: 02/07/2014

ISSN (print): 1744-3091

ISSN (electronic): 2053-230X

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1107/S2053230X14015520

DOI: 10.1107/S2053230X14015520


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