Lookup NU author(s): Thabata Alvarez,
Professor Harry Gilbert
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In recent years, owing to the growing global demand for energy, dependence on fossil fuels, limited natural resources and environmental pollution, biofuels have attracted great interest as a source of renewable energy. However, the production of biofuels from plant biomass is still considered to be an expensive technology. In this context, the study of carbohydrate-binding modules (CBMs), which are involved in guiding the catalytic domains of glycoside hydrolases for polysaccharide degradation, is attracting growing attention. Aiming at the identification of new CBMs, a sugarcane soil metagenomic library was analyzed and an uncharacterized CBM (CBM_E1) was identified. In this study, CBM_E1 was expressed, purified and crystallized. X-ray diffraction data were collected to 1.95 angstrom resolution. The crystals, which were obtained by the sitting-drop vapourdiffusion method, belonged to space group I23, with unit-cell parameters a = b = c = 88.07 angstrom.
Author(s): Campos BM, Alvarez TM, Liberato MV, Polikarpov I, Gilbert HJ, Zeri ACD, Squina FM
Publication type: Article
Publication status: Published
Journal: Acta Crystallographica Section F: Structural Biology Communications
Print publication date: 01/09/2014
Online publication date: 27/08/2014
Acceptance date: 02/07/2014
ISSN (print): 1744-3091
ISSN (electronic): 2053-230X
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