Lookup NU author(s): Dr Steve Boneham
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Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis.
Author(s): McClure P, Curran R, Boneham S, Ball JK
Publication type: Article
Publication status: Published
Journal: Journal of Virological Methods
Print publication date: 26/07/2000
Online publication date: 27/07/2000
Acceptance date: 06/04/2000
ISSN (print): 0166-0934
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