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Glucose Oxidation Using Oxygen Resistant Pyranose-2-Oxidase for Biofuel Cell Applications

Lookup NU author(s): Samet ┼×ahin, Dr Eileen Yu

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Abstract

In this study, the effect of oxygen on glucose oxidation using Glucose Oxidase (GOx) and oxygen resistant Pyranose-2-Oxidase (P2O) has been studied. Enzyme solutions with ferrocene carboxylic acid (FcCOOH) as electron mediator were tested with glassy carbon electrode (GCE) under air and nitrogen saturated conditions in a three electrode electrochemical cell system. Electrochemical characterization of enzymes has been achieved in solution by using cyclic voltammetry (CV), linear sweep voltammetry (LSV) and chronoamperometry (CA). In the presence of glucose, CV and LSV results show increasing anodic peak current and decreasing cathodic peak current with increasing glucose concentrations, which reflects the ferrocene-mediated bioelectrocatalysis of glucose oxidation. The experiments with CA show enhanced stability with oxygen resistant P2O where GOx loses 30 % of its current density in the presence of oxygen after 3 hours. These results indicate that P20, a promising enzyme with no oxygen reactivity and long stability, which can be used in enzymatic biofuel cell applications as an alternative to GOx.


Publication metadata

Author(s): Sahin S, Wongnate T, Chaiyen P, Yu EH

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: 10th European Symposium on Electrochemical Engineering

Year of Conference: 2014

Pages: 367-372

Print publication date: 01/01/2014

ISSN: 1974-9791

Publisher: AIDIC Servizi

URL: http://dx.doi.org/10.3303/CET1441062

DOI: 10.3303/CET1441062

Library holdings: Search Newcastle University Library for this item

Series Title: Chemical Engineering Transactions

Sponsor(s): Italian Assoc Chem Engn

ISBN: 9788895608327


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