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Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258^T) to 97.8 % (Actinokineospora baliensis NBRC 104211^T). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H₄). The major fatty acids were found to be iso-C_16:0, iso-C_15:0, iso-C_16:1 H and iso-C_16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84^T (=DSM 45961^T = CECT 8578^T).

Publication metadata

Author(s): Aouiche A, Bouras N, Mokrane S, Zitouni A, Schumann P, Spröer C, Sabaou N, Klenk HP

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2015

Volume: 107

Issue: 1

Pages: 291-296

Print publication date: 01/01/2015

Online publication date: 07/11/2014

Acceptance date: 01/11/2014

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10482-014-0328-8

DOI: 10.1007/s10482-014-0328-8

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