Lookup NU author(s): Dr Maria Del Carmen Montero-Calasanz,
Professor Hans-Peter Klenk
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15–35°C, at pH 5.5–9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1ω8c and C16:1ω7c. The 16S rRNA gene showed 97.4–99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18T (= DSM 44209T = CCUG 63018T). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.
Author(s): Montero-Calasanz MDC, Hofner B, Goker M, Rohde M, Sproer C, Hezbri K, Gtari M, Schumann P, Klenk HP
Publication type: Article
Publication status: Published
Journal: BioMed Research International
Online publication date: 09/07/2014
Acceptance date: 09/06/2014
Date deposited: 18/03/2015
ISSN (print): 2314-6133
ISSN (electronic): 2314-6141
Publisher: Hindawi Publishing Corporation
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