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Geodermatophilus poikilotrophi sp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

Lookup NU author(s): Dr Maria Del Carmen Montero-Calasanz, Professor Hans-Peter Klenk

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15–35°C, at pH 5.5–9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1ω8c and C16:1ω7c. The 16S rRNA gene showed 97.4–99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18T (= DSM 44209T = CCUG 63018T). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.


Publication metadata

Author(s): Montero-Calasanz MDC, Hofner B, Goker M, Rohde M, Sproer C, Hezbri K, Gtari M, Schumann P, Klenk HP

Publication type: Article

Publication status: Published

Journal: BioMed Research International

Year: 2014

Online publication date: 09/07/2014

Acceptance date: 09/06/2014

Date deposited: 18/03/2015

ISSN (print): 2314-6133

ISSN (electronic): 2314-6141

Publisher: Hindawi Publishing Corporation

URL: http://dx.doi.org/10.1155/2014/914767

DOI: 10.1155/2014/914767


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