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Lookup NU author(s): Dr Miguel Velazquez
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Analysis of luminal fluid microenvironments in the reproductive tract is pivotal to elucidate embryo-maternal signaling mechanisms responsible for successful reproduction in mammals, including cattle. Besides facilitating production of an optimized medium for in vitro fertilization and embryo culture in assisted reproductive technologies, screening of luminal fluid constituents in the oviduct and uterus could also provide critical information for elucidation of mechanisms underlying developmental programming. A key issue in this type of research is the sampling of luminal fluids. In this review we discuss the sampling techniques available for bovine species, including a recent in situ technique developed with the Ghent device, which allows rapid recovery of measurable amounts of pure uterine luminal fluid with minimal disturbance to the donor animal.
Author(s): Velazquez MA, Parrilla I, VanSoom A, Verberckmoes S, Kues W, Niemann H
Publication type: Review
Publication status: Published
Journal: Theriogenology
Year: 2010
Volume: 73
Issue: 6
Pages: 758-767
Print publication date: 01/04/2010
Online publication date: 13/08/2009
Acceptance date: 28/04/2009
ISSN (print): 0093-691X
ISSN (electronic): 1879-3231
Publisher: Elsevier
URL: . https://doi.org/10.1016/j.theriogenology.2009.07.004
DOI: 10.1016/j.theriogenology.2009.07.004
